Gebze Technical University

Cancer Molecular Genetics

Laboratory Name	Cancer Molecular Genetics
Laboratory Responsible	Assist. Prof. Can M. Erişti
Department	Molecular Biology and Genetics
Telephone	+90 (262) 6052548/6052517
Address	Gebze Institute of Technology Department of Molecular Biology and Genetics C-2/221 41400 Cayirova, Kocaeli, TURKEY
General Information	Research in our laboratory focuses on the regulation of gene expression in eukaryotic cells. Current experiments in the lab are directed at understanding the molecular mechanisms of isoflavone-induced downregulation of certain genes in prostate cancer cells. Our aim is not only to obtain further insights on the molecular mechanisms through which isoflavones exert their effect in cancerous cells, but to provide contribution on the context of development of more effective chemopreventive and therapeutic strategies.
Laboratory Staff	Zeynep Özcan (PhD) Mehmet Sarıhan (MS) Sinem Gündüz (MS)
Applications	1. Reporter gene assays to identify important sites in gene promoters 2. siRNA knockdown in cell culture to elucidate gene function 3. qRT-PCR to determine level of gene expression 4. Fluorescent microscopy to detect intracellular localization of proteins 5. 2D gel electrophoresis to analyze protein expression patterns 6. Western blotting to detect specific proteins
Equipment	1. Varioskan Flash Multimode Reader (Thermo) 2. LightCycler 480 System (Roche) 3. Protean IEF System (Bio-Rad)