Laboratory Name

 Biochemistry Laboratory

Laboratory Responsible

Prof.Dr. Aziz TANRISEVEN

Department

Chemistry

Telephone

+90 (262) 6053070 - 6053064

Address

Gebze Institute of Technology, Faculty of Science, Chemistry Department, Muallimköy Campus, P.O.Box 141 Gebze-Kocaeli, 41400, TURKEY 

General Information

The Studies are focused on;1) Discovering of novel enzymes and large scale recombinant production2) Biochemical characterization of enzymes 3) Enzyme immobilization studies4) Biotechnological process 

Laboratory Staff

Prof. Dr. Aziz Tanrıseven, Assoc. Prof. Ali Türkan, Assoc. Prof. Ayhan Çelik, Dr. Zehra Ölçer PhD Students:Research Assistant Aişe Ünlü, Gülden Yetiş, Fatma Ertan, Şaban Kalay, Fatih Aktaş, Mahmut Parlak, İlknur Aksoy, Murat Çelik,MSc Students:Serkan Akköse, Serhan Şen, Kübra Ekşiler, Arzu Gürbüz, Damla Kabil, N.Nazmiye Gezer, Derya Günal, Meral Amanvermez Karabaş, Özgür Gökmen, Özden Doruköz

Applications

Recombinant DNA Technologies: Discovering of novel enzymesElectrophoresis: Analysis of DNA and proteinsEnzyme assays: Determination of enzyme activity using spectrophotometric techniquesEnzyme Characterization: pH, Temperature, susbtrate specificity and kineticsEnzyme immobilization: Enzyme immobilization and characterizationFermentation Technologies: Bacterial fermentationProtein mass determination: Determination of proteins mass with MALDI-TOFProdrug/Enzyme systems

Equipment

Centrifuges (Beckman Coulter Allegra 64R , Nüve NF 1200 R) 1.JPEG 2.JPEGUV-VIS-NIR Spectrophotometers (Shimadzu 3600) 3.JPEGMicroplate Reader (Spectramax PLUS ) 4.JPEGpH Meter (InoLab)Incubator(New Brunswick Scientific Excella 25) 5.JPEGOrbital Shaker (IsoLab Shaker SH3D) 6.JPEGMicrocentrifuges (Wise Spin CF-10) 7.JPEGAutoclave (Varioklav) 8.JPEGSDS-PAGE (Bio-Rad) and Agarose (ThermoScientific) Gel Electrophoresis 9.JPEGPCR (Finnzymes, Applied Biosystems Veriti 96 Well Thermal Cycler)10.JPEG 11.JPEG-80°C freezer (New Brunswick Scientific Ultra Low Temperature Freezer)12.JPEGLaminar flow cabinet 13.JPEGU.V Transilluminator 14.JPEGDensitometer (BioRad GS-670) 15.JPEGFreeze drier 16.JPEGIce machine (İzmak) 17.JPEGVortex (Stuart Vortex mixer) 18.JPEG

Projects

1. “Production of Isomaltooligosaccharides from Sucrose Using Membrane Bioreactors” Tübitak Project No: 106M001 (2005-2007) 2. “Investigation of Activities and Expressions of Pyruvate Dehydrogenase Complex and Pyruvate Dehydrogenase Kinase and Phosphatase Isoenzymes in Breast Cancer and Malignant Mesothelioma Cells” Tübitak Project No: 108T910 (2009-2011)3. “Development of Nitroreductase Prodrug Systems for Cancer theraphy” Tübitak Project No: 110T754 (2011-2013)4. “Biotechnological Treatment of Biowastes for the Production of High Value Added Products” Tübitak Project No: 109Y372 (2011-2013)