Laboratory Name

Gebze Enzyme Recognition Center, Binay Research Laboratory 

Laboratory Responsible

 Asst. Prof. Dr. Barış Binay

Department

 Department of Bioengineering

Telephone

Address

Gebze Technical University, Engineering Faculty, Lab Number:142
41400 Çayırova Gebze/KOCAELİ

http://www.gtu.edu.tr/gerc/home-page.html

Laboratory Staff

Mervan Çakar
Meltem ÇORBACIOĞLU
Uğur PALA
Ersin KARATAŞ
Bedri BURAK DURAKSOY
Ahmet TÜLEK
Berin YELMAZER
Ünzile GÜVEN GÜLHAN
Ece Özdemir

General Information

Applications

Kinetic and activity measurements of enzymes

Recognitions of novel or increased stability of enzymes

Genomic and proteomic analyses

Gene and protein synthesis

Equipment

1. Heater Magnetic mixer (Wisesir)
2. Hotplate Stuart (CB300)
3. Digital pH meter (Hanna H2211)
4. PCR (Techne Prime)
5. Mini centrifuge/ refrigerated (Sigma 1-16K)
6. Wortex Mixer (N-Biotek NB105)
7. Scales (WLC RADWAG,WTB 2000)
8. Multiwell Plate Reader (Biotek- ELx808)
9. SDS-PAGE Electrophoresis and Power Supply (Biorad)
10. Benchtop Centrifuge (Hettich Rotina 380R)
11. Single and multi pipetors (Axygen)
12. Etuve (Nüve EN500)
13. Vacuum Pump (Gm-0.5, Isolab)
14. Horizontal Electrophoresis Tank (Bluemarıne, Serva)
15. Ultrasonic Homogenizer (Uw 2070, Bandelin)
16. Biosafety Class II Cabin (Nb-604 Ws 90, N-Bıotek)
17. Gel Screening (Bluelight, Serva)
18. Ultracentrifuge (Allegra X-30r, Beckman)
19. Ultrahigh freezer (-80 °C) (Ultf -80, Arctiko)
20. Deionized Water and Distilled Water (Millipore Direct Q 5UV)
21. Fridge +4°C and -20°C (Vestel)
22. Magnetic mixer (IKA RH Basic 2)
23. Precision Balance (SHIMADZU ATX224)
24. Precision Balances (AND HR 250AZ)
25. Benchtop shaking incubator (N-Biotek NB205-L)
26. Plate shaker (Stuart SSM5)
27. Block Heater (Stuart 130D/3)
28. Tube heater Benchmark (Multi-Therm)
29. Ice Machine (IMS-50)
30. Centrifuge refrigerated (Heithtech Rotina 380R)
31. Centrifuge refrigerated (Bechman Colture Allegra X30-R)

1. COST- 214Z292, (01.06.2015 - 01.06.2018), Production of Renewable Energy from Carbon Dioxide (CO2) with the Multidimensional Mutational Analysis of the Active Region of the Format Dehydrogenase Enzymes, 454.020 TL

2. CMST COST Action CM1303, (2014 - 2018), Systems Biocatalysis Work Group 4, 1.7 Milyon Euro

3. TÜBİTAK-3501/214Z064, (01.05.2015 – 01.05.2018), For ensuring continuous renewal of NAD(P)H for asymmetric synthesis in industrial biocatalytic processes isolation and characterization of the heat-resistant NAD(P) +-dependent format dehydrogenase from Chaetomium thermophilum, 312.448 TL

4. TÜBİTAK-1002/114Z798, (15.01.2015 - 15.01.2016), Based on the understanding the of the active region architecture, increasing specific activity of NAD+ dependent mutant of Candida methylica format dehydrogenase, 29.595 TL

5. TUBİTAK-3001/213S099, (01.04.2014 – 01.04.2016), Designing bsLDH based on iterative saturation mutagenesis (ISM) for asymmetric synthesis industrially important chiral α-hydroxy acids, 75.560 TL

6. DPT-90188 (01.01.2006 – 01.01.2008), 547.000 TL