Page 81 - ARAŞTIRMA ÖZETLERİ

LİSANSÜSTÜ ARAŞTIRMALAR SEMPOZYUMU & TANITIM GÜNLERİ 2018

Influence of Pseudomonas Putida on in-vitro Seedling Growth of Arabidopsis Thaliana

Ebru ARSLAN, Yelda ÖZDEN ÇİFTÇİ, Özlem AKKAYA
Moleküler Biyoloji ve Genetik ABD

ÖZET
Arabidopsis thaliana (A. thaliana) is a model organism due to its small genome that has been sequenced in

the year 2000, a short generation time, small size and prolific seed production through self‐pollination. Beneficial
microbes, which are known as plant growth promoting microorganisms (PGPM), and plants can establish a
multitude of interactions with one another in natural and agricultural environments. Among them, Pseudomonas
putida (P. putida) which is a Gram-negative, aerobic, rod shaped bacterium could be a promising candidate to
understand plant-microbe interactions. Therefore, the aim of this study concerns the evaluation of the effects of P.
putida on the in vitro seedling growth of A. thaliana. Different bacterial concentrations from 2.5x105-2.5x107
CFU/ml were used for 14 days old in vitro germinated seedlings. Then, morphological and physiological analysis
were carried out to determine plant and bacteria interaction. Our results demostrated that inoculation of A.
thaliana with a bacterial concentration of 2.5x107 CFU/ml was better than others. Our findings provide a basis for
extensive further studies together with a significant advance in understanding of plant-microbe interactions.
Moreover, it will help to achieve bioremediation through plant-bacterial associations for future studies.
Anahtar Kelimeler: Arabidopsis, Pseudomonas Putida, Plant-Microbe İnteractions, Plant Growth-Promoting
Microorganisms (PGPM).

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