Webinar #47 Enzyme-DNA binding protein conjugates for signal generation and amplification in biosensors
Enzyme-DNA binding protein conjugates for signal generation and amplification in biosensors
Dr. Gülsen Betül AKTAŞ
Bilgi University
27th May2022, 11:00 (Turkish Time)
Summary
Proteins are the primary functional agents that play essential roles in life processes. Since their discovery, proteins had a big impact in the different research areas especially as the building blocks of biosensors. The field of the biosensors has been hailed as the detection to many diseases in health industry. The protein immobilization mediated the improvement of biosensors from bottom to top; surface immobilization to detection element. The research on robust methods for the controlled protein co-immobilization has become essential on the recent years. Therefore, novel methods for the co-immobilization methods of desired proteins and nanoparticles for improved biosensors are under investigation. In this presentation, the use of DNA binding proteins in biosensors for signal generation and amplification is explained. The specificity and high affinity that these proteins exhibit for their respective double stranded DNA (dsDNA) sequence was thus exploited in order to develop a protein immobilization technique that allows the controlled co-immobilization of several proteins. The system is based on the utilization of these DNA binding proteins as fusion tags for the target proteins to be immobilized and the use of dsDNA as the template to direct the protein co-immobilization by specific positioning of the target DNA sequences recognized by the DNA binding proteins. The preparation and characterization of two novel universal enzyme-DNA binding protein conjugates is described and their use for signal generation and amplification in biosensors for the detection of nucleic acid and protein targets is demonstrated. In addition, the suitability of these DNA binding proteins to directly immobilize DNA molecules is also shown.
